Dogra, ShilpaO’Rourke, Nicholas2022-08-292022-08-292022-08-01https://hdl.handle.net/10155/1499The effect of prolonged sitting on inflammation, as determined by cytokine profiles, is relatively unexplored. The purpose of this work was to determine the reliability of salivary IL-8 (CXCL8) and the response of IL-8 to prolonged and interrupted sitting. Participants completed two sessions in random order: prolonged sitting (4 hours of sitting) and interrupted sitting (prolonged sitting interrupted every 30 minutes by 3 minutes of walking). Saliva and capillary plasma samples were collected pre- and post-session. Weekly variability in salivary IL-8 and individual responses were also explored. Prolonged sitting increased salivary IL-8 concentrations, while interrupted sitting attenuated this response. Among males, there was no increase in salivary IL-8 during interrupted sitting. Prolonged sitting may serve as a pro-inflammatory stimulus, and movement interruptions may negate this response. Saliva sampling seems to be a reliable method in assessing IL-8 across timepoints within individuals; however, there is high variability across individuals.enSedentary behaviourInflammationCytokinesInterleukin 8SittingThesis