Using non-targeted mass spectrometry to identify proteomic, metabolomic and lipidomic profiles in saliva to compare the impact of prolonged vs. interrupted sitting
Date
2024-08-01
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Abstract
Traditional sedentary physiology research focuses on targeted approaches. To explore from a new perspective, a non-targeted approach with the use of Liquid Chromatography-Mass Spectrometry (LC-MS) to assess the responsiveness of proteins and metabolites in saliva to sedentarism was employed. The study involved 24 participants that engaged in both a prolonged sitting session and an interrupted sitting session, in a randomized order. Saliva samples were collected before and after each session, including a separate baseline. Samples were purified and concentrated then analyzed on the LC-MS. A total of 2493 proteins, 17 lipids, and 11 biogenic amines were detected. 2112 proteins were significantly differentially abundant among the different session sample groups. In conclusion, prolonged sedentary behaviour of 4 hours led to a range of molecular responses that were observed through changes in the relative abundances of proteins and lipids. The results were complex and suggestive of several indicators of health deterioration.