The synthesis and in vitro evaluation of short interfering RNA containing triazole functionalized sphingolipid-based derivatives

dc.contributor.advisorDesaulniers, Jean-Paul
dc.contributor.authorFernandez, Charlene A.
dc.date.accessioned2022-02-07T21:07:53Z
dc.date.accessioned2022-03-29T17:27:19Z
dc.date.available2022-02-07T21:07:53Z
dc.date.available2022-03-29T17:27:19Z
dc.date.issued2021-12-01
dc.degree.disciplineApplied Bioscience
dc.degree.levelMaster of Science (MSc)
dc.description.abstractRNA Interference (RNAi) is a natural cellular process that silences the expression of a target gene in a sequence-specific manner by mediating targeted mRNA degradation. One of the main challenges in RNAi research is developing an effective delivery system for siRNAs. Recently, fatty acid conjugated siRNAs have shown promising potential as safe and effective method to deliver oligonucleotides to extrahepatic cells. A class of fatty acids that we are interested in are sphingolipids, they are a class of lipids containing a backbone of sphingoid bases and aliphatic amino alcohols. Sphingolipids show potential as lipid-conjugated siRNAs as they are important structural and functional components of plasma membranes of eukaryotic cells. Here, we design a library of siRNAs containing propyl-triazole modified sphingosine at various locations of the sense and antisense strand of the reporter gene firefly luciferase and evaluate its gene-silencing abilities in both the presence and absence of a transfection carrier.en
dc.description.sponsorshipUniversity of Ontario Institute of Technologyen
dc.identifier.urihttps://hdl.handle.net/10155/1414
dc.language.isoenen
dc.subjectSphingolipidsen
dc.subjectsiRNAen
dc.subjectGene-silencingen
dc.subjectCellular uptakeen
dc.subjectRNA interferenceen
dc.titleThe synthesis and in vitro evaluation of short interfering RNA containing triazole functionalized sphingolipid-based derivativesen
dc.typeThesisen
thesis.degree.disciplineApplied Bioscience
thesis.degree.grantorUniversity of Ontario Institute of Technology
thesis.degree.nameMaster of Science (MSc)

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