Silence of the fish: injection of photoswitchable short interfering RNA oligonucleotides into Japanese medaka embryos (oryzias latipes) to photochemically control gene silencing

dc.contributor.advisorDesaulniers, Jean-Paul
dc.contributor.advisorSimmons, Denina
dc.contributor.authorMateus, Makenzie
dc.date.accessioned2024-01-23T20:44:04Z
dc.date.available2024-01-23T20:44:04Z
dc.date.issued2023-12-01
dc.degree.disciplineApplied Bioscience
dc.degree.levelMaster of Science (MSc)
dc.description.abstractThe siRNA duplex functions by binding to and cleaving mRNA, a process known as the RNAi pathway. Existing siRNAs face challenges such as off-target effects and unpredictable prolonged gene-silencing. To address these issues, enhancing their therapeutic potential, siRNAs can be modified controlling their role in the RNAi pathway. An Ortho-functionalized, tetrafluorinatedazobenzene was integrated into the siRNA backbone via phophoramidite chemistry to generate a class of photoswitchable F-siRNAs. These F-siRNAs retained the ability to photoisomerize from an active trans to an inactive cis state through blue and green light respectively. This thesis reported on a novel technique to inject single cell medaka embryos with both Wt and F-siRNAs. Medaka are a small freshwater teleost fish that have a number of desirable features for use as a vertebrate model in gene silencing projects. These attributes include daily spawning, a number of useful genetic strains, a completely sequenced genome, and a transparent egg.en
dc.description.sponsorshipUniversity of Ontario Institute of Technologyen
dc.identifier.urihttps://hdl.handle.net/10155/1727
dc.language.isoenen
dc.subjectsiRNAen
dc.subjectAntisenseen
dc.subjectJapanese medakaen
dc.subjectNano injectionen
dc.subjectTransgenicen
dc.titleSilence of the fish: injection of photoswitchable short interfering RNA oligonucleotides into Japanese medaka embryos (oryzias latipes) to photochemically control gene silencingen
dc.typeThesisen
thesis.degree.disciplineApplied Bioscience
thesis.degree.grantorUniversity of Ontario Institute of Technology
thesis.degree.nameMaster of Science (MSc)

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